- Abstract:
-
Draft sequencing is a rapid and efficient method for determining the near-complete sequence of microbial genomes. Here we report a comparative analysis of one complete and two draft genome sequences of the phytopathogenic bacterium, Xylella fastidiosa, which causes serious disease in plants, including citrus, almond, and oleander. We present highlights of an in silico analysis based on a comparison of reconstructions of core biological subsystems. Cellular pathway reconstructions have been used to identify a small number of genes, which are likely to reside within the draft genomes but are not captured in the draft assembly. These represented only a small fraction of all genes and were predominantly large and small ribosomal subunit protein components. By using this approach, some of the inherent limitations of draft sequence can be significantly reduced. Despite the incomplete nature of the draft genomes, it is possible to identify several phage-related genes, which appear to be absent from the draft genomes and not the result of insufficient sequence sampling. This region may therefore identify potential host-specific functions. Based on this first functional reconstruction of a phytopathogenic microbe, we spotlight an unusual respiration machinery as a potential target for biological control. We also predicted and developed a new defined growth medium for Xylella.
- Links To Paper
- 1st Link
- Bibtex format
- @Article{EDI-INF-RR-1163,
- author = {
Anamitra Bhattacharyya
and Stephanie Stilwagen
and Gary Reznik
and Helene Feil
and William S. Feil
and Iain Anderson
and Evgeny Selkov
and Axel Bernal
and Mark D'Souza
and Natalia Ivanova
and Vinayak Kapatral
and Niels Larsen
and Tamara Los
and Athanasios Lykidis
and Theresa L. Walunas
and Alexander Purcell
and Rob A. Edwards
and Trevor Hawkins
and Robert Haselkorn
and Ross Overbeek
and Nikos C. Kyrpides
and Paul F. Predki
},
- title = {Draft sequencing and comparative genomics of Xylella fastidiosa strains reveal novel biological insights},
- journal = {Genome Research},
- publisher = {Genome Research},
- year = 2002,
- month = {Oct},
- volume = {12(10)},
- pages = {1556-1563},
- url = {http://www.genome.org/cgi/reprint/12/10/1556?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=1&author1=selkov&andorexacttitle=and&andorexacttitleabs=and&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT},
- }
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